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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
02/02/2023 |
Data da última atualização: |
24/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CÔRTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H. |
Afiliação: |
KATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK. |
Título: |
A Mad7 system for genetic engineering of filamentous fungi. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Fungi, v. 9, n. 1, 16, 2023. |
ISSN: |
2309-608X |
DOI: |
https://doi.org/10.3390/jof9010016 |
Idioma: |
Inglês |
Conteúdo: |
The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. |
Palavras-Chave: |
CRISPR; Filamentous fungi; Fungal strain engineering; Mad7. |
Thesagro: |
Fungo. |
Thesaurus Nal: |
Aspergillus; Fungi; Genetic engineering. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151460/1/jof-2023.pdf
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Marc: |
LEADER 02290naa a2200325 a 4500 001 2151460 005 2023-03-24 008 2023 bl uuuu u00u1 u #d 022 $a2309-608X 024 7 $ahttps://doi.org/10.3390/jof9010016$2DOI 100 1 $aVANEGAS K. G. 245 $aA Mad7 system for genetic engineering of filamentous fungi.$h[electronic resource] 260 $c2023 520 $aThe introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. 650 $aAspergillus 650 $aFungi 650 $aGenetic engineering 650 $aFungo 653 $aCRISPR 653 $aFilamentous fungi 653 $aFungal strain engineering 653 $aMad7 700 1 $aRENDSVIG, J. K. H. 700 1 $aJARCZYSKA, Z. D. 700 1 $aCÔRTES, M. V. de C. B. 700 1 $aVAN ESCH, A. P. 700 1 $aMORERA-GÓMEZ, M. 700 1 $aCONTESINI, F. J. 700 1 $aMORTENSEN, U. H. 773 $tJournal of Fungi$gv. 9, n. 1, 16, 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Roraima. |
Data corrente: |
06/10/2008 |
Data da última atualização: |
07/02/2020 |
Autoria: |
OLIVEIRA JUNIOR, M. C. M. de; BENDAHAN, A. B.; XAUD, M. R. |
Afiliação: |
MOISES CORDEIRO MOURAO DE O JUNIOR, CPAF-RR; AMAURY BURLAMAQUI BENDAHAN, CPAF-RR; MARISTELA RAMALHO XAUD, CPAF-RR. |
Título: |
Aproximação de modelo determinístico para estimação diária de umidade relativa do ar na região do Apiaú , Roraima |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Boa Vista: Embrapa Roraima, 2006. |
Série: |
(Embrapa Roraima. Boletim de Pesquisa e Desenvolvimento, 07) |
Idioma: |
Português |
Palavras-Chave: |
Agrometeorologia; Roraima; Série temporais. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174237/1/bp072006-pluviusAP-amaury.pdf
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Marc: |
LEADER 00581nam a2200169 a 4500 001 1693095 005 2020-02-07 008 2006 bl uuuu u0uu1 u #d 100 1 $aOLIVEIRA JUNIOR, M. C. M. de 245 $aAproximação de modelo determinístico para estimação diária de umidade relativa do ar na região do Apiaú , Roraima 260 $aBoa Vista: Embrapa Roraima$c2006 490 $a(Embrapa Roraima. Boletim de Pesquisa e Desenvolvimento, 07) 653 $aAgrometeorologia 653 $aRoraima 653 $aSérie temporais 700 1 $aBENDAHAN, A. B. 700 1 $aXAUD, M. R.
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Embrapa Roraima (CPAF-RR) |
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